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Author Lo, Chia-Feng ♦ Yu, Chi-Yang ♦ Kuan, I.-Ching ♦ Lee, Shiow-Ling
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Burkholderia Sp ♦ Lipase Production ♦ Response Surface Methodology ♦ Olive Oil ♦ Optimized Medium ♦ Optimum Concentration ♦ Preliminary Test ♦ Extracellular Lipase Production ♦ Basal Medium ♦ Predicted Value ♦ Faced-centered Central Composite Design ♦ Maximum Activity ♦ Significant Effect
Abstract Abstract: Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were determined using a faced-centered central composite design (FCCCD). The analysis of variance revealed that the established model was significant (p < 0.01). The optimized medium containing 0.65 % olive oil (v/v), 2.42 % tryptone (w/v) and 0.15 % Tween-80 (v/v) resulted in a maximum activity of 122.3 U/mL, about three fold higher than that in basal medium. Approximately 99 % of validity of the predicted value was achieved.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2012-01-01