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Author Snijders, Peter J. F. ♦ Steenbergen, Renske D. M. ♦ Top, Bert ♦ Scott, S. Imon D. ♦ Jan M. M. Walboomers, I.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Tonsillar Carcinoma ♦ Human Papillomavirus ♦ P53 Status ♦ P53 Immunohistochemistry ♦ Hpv Dna ♦ Gp-pcr Product ♦ Tonsillar Squamous Cell Carcinoma ♦ Subsequent Typing ♦ Hpv Type-specific Pcr ♦ P53 Mutation ♦ Single Case ♦ P53 Alteration ♦ Hybridization Analysis ♦ Polyclonal Serum Cm-1 ♦ P53 Level
Abstract Tonsillar squamous cell carcinomas (a total of 14) were examined both for the presence of human papillomavirus (HPV) DNA and for p53 alterations. General primer-mediated HPV polymerase chain reaction (GP-PCR) revealed the presence of HPV DNA in 12/14 cases. Subsequent typing by HPV type-specific PCR and sequence or hybridization analysis of GP-PCR products revealed DNA from HPV 16 in seven cases, from HPV 33 in two cases, and from HPV 7, HPV 16/33 and HPV 33/59 each in a single case. p53 immunohistochemistry performed on nine HPV containing tonsillar carcinomas using polyclonal serum CM-1 showed elevated p53 levels in four cases. These included 3/5 HPV 16 containing carcinomas and the HPV 33/59 containing carcinoma. Analysis of p53 mutations using denaturing
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study