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Author Shi, Weibin ♦ Brown, Morry D. ♦ Wang, Xuping ♦ Wong, Jack ♦ Kallmes, David F. ♦ Matsumoto, Alan H. ♦ Helm, Gregory A. ♦ Drake, Thomas A. ♦ Lusis, Aldons J.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Apoe Mouse ♦ Aortic Root ♦ Genetic Background ♦ Atherosclerotic Lesion Determine Medial Destruction ♦ Apolipoprotein Deficient Mouse ♦ Atherosclerotic Lesion ♦ Elastic Medium ♦ Aortic Tissue ♦ Medial Destruction ♦ Apolipoprotein Deficient ♦ Atherosclerotic Aortic Wall ♦ Striking Histologic Feature ♦ Mmp Proteolytic Activity ♦ Apoe Strain ♦ Atherosclerotic Aortic Aneurysm ♦ Atherosclerosis Genetic Predisposition Aortic Aneurysm ♦ Mmp-9 And-12 ♦ Western Diet Develop ♦ Focal Dilatation Beneath Plaque ♦ Result C3h ♦ Matrix Metalloproteinases ♦ Arterioscler Thromb Vasc Biol ♦ C3h Hej ♦ Objective Destruction ♦ Casein Zymography ♦ Aortic Wall ♦ Western Diet ♦ Proteolytic Activity ♦ Atherosclerosis Susceptibility ♦ Mmp-2 And-12 Activity ♦ Mmp-9 Activity ♦ Conclusion Data Indicate
Abstract Objective—Destruction of the elastic media is the most striking histologic feature of atherosclerotic aortic aneurysms. Apolipoprotein E–deficient (apoE-/-) mice fed a Western diet develop advanced atherosclerotic lesions in the aorta. We sought to assess the integrity of atherosclerotic aortic walls in 2 apoE-/- strains, C57BL/6 (B6) and C3H/HeJ (C3H) that differ markedly in atherosclerosis susceptibility. Methods and Results—C3H.apoE-/- mice developed much smaller atherosclerotic lesions than did B6.apoE-/- mice after being fed a Western diet for 16 weeks, but the C3H.apoE/ mice exhibited destruction of the elastic media, including erosion, fragmentation, and focal dilatation beneath plaques. Gelatin and casein zymography showed proteolytic activity of matrix metalloproteinases (MMPs)-9,-2, and-12 in aortic tissues and of MMP-9 and-12 in macrophages from both strains. However, C3H.apoE-/- mice showed significantly increased MMP-2 and-12 activity in aortas and macrophages compared with those from B6.apoE-/- mice. MMP-9 activity was comparable in aortic tissues of the 2 strains, but it was significantly higher in macrophages from C3H.apoE-/- than from B6.apoE-/- mice. Conclusions—Data indicate that genetic backgrounds but not sizes of atherosclerotic lesions determine medial destruction in the aortic root of apoE-/- mice and that an increase in MMP proteolytic activity might contribute to the medial destruction of aortic walls in C3H.apoE-/- mice. (Arterioscler Thromb Vasc Biol. 2003;23:1901-1906.) Key Words: atherosclerosis genetic predisposition aortic aneurysms matrix metalloproteinases
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study