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Author Reactors, Glutamate Dehydrogenase ♦ Stalikas, C. D. ♦ Karayannis, M. I. ♦ Tzouwara-Karayanni, Stella M.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Human Serum ♦ Flow Injection Determination ♦ Bed Reactor ♦ Single Bead String Reactor ♦ Measuring System ♦ Mammalian Central Nervous System ♦ Fluorometric Detection ♦ Gas Chromatogra-glutamic Acid ♦ Optimal Dilution Factor ♦ Serum Sample ♦ Rat Brain ♦ Glutamate Oxidase ♦ Non-porous Glass Bead ♦ Glass Derivative ♦ Glutamate Dehydrogenase ♦ Brain Sample ♦ Between-day Measurement ♦ Flow Injection Analysis System ♦ Glutamic Acid ♦ Relative Standard Deviation ♦ High Performance Liquid Chromatography ♦ Optimal Dilution Ratio ♦ Regional Concentration ♦ Method Comparison Study ♦ Important Neuro Phymass Spectrometry
Abstract Summary: Two methods are proposed for the determination of regional concentrations of glutamate in the rat brain äs well äs in human serum. Glutamate oxidase was immobilized on non-porous glass beads and glutamate dehydrogenase was immobilized on glass derivatives. These Supports were employed for the construction of Single Bead String Reactors and Packed Bed Reactors, respectively, which in turn were linked to Flow Injection Analysis Systems with either photometric or fluorometric detection. Analytical working curves are linear in the ränge 1—200 / for packed bed reactors and 10—500 mmol/1 for single bead string reactors. The samples were pretreated depending on their origin and the applied measuring System. Optimal dilution factors were established for the two techniques. Optimal dilution ratios were established and the influence of several added substances was investigated. Recovery and method comparison studies including high performance liquid chromatography verified the accuracy of the proposed methods. Results frorn within-day and between-day measurements gave relative Standard deviations of 4.7 and 5.9 % for serum samples and 2.5 and 4.0 % for brain samples, respectively. Introduction enzymes (8—11), HPLC (12, 13) and gas chromatogra-Glutamic acid is considered to be an important neuro- Phymass spectrometry (14, 15). All these methods have transmitter in the mammalian central nervous System (1) been aPPhed for the determination of glutamic acid m
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Publisher Date 1994-01-01