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Author Freund, Jean-Noël ♦ Clevers, Hans
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Sox9 Intestine Crypt Transcription Factor ♦ Intestinal Epithelium ♦ Intestine Epithelium ♦ Colon Epithelium-derived Cell ♦ Mature Villus Cell ♦ Mouse Fetal Intestine ♦ Transcriptional Effector ♦ Sox Expression Screening ♦ Hmg Box Transcription Factor Family ♦ Wnt Pathway ♦ Whereas Tcfs ♦ Progenitor Cell Phenotype ♦ Pattern Characteristic ♦ Bipartite Catenin Tcf4 Transcription Factor ♦ Sox9 Protein ♦ Wnt-dependent Maintenance ♦ Vivo Evidence ♦ Epithelial Cell ♦ Muc2 Gene ♦ Sox9 Expression ♦ Wnt Target
Abstract TCF and SOX proteins belong to the HMG box transcription factor family. Whereas TCFs, the transcriptional effectors of the WNT pathway, have been widely implicated in the development, homeostasis and disease of the intestine epithelium, little is known about the function of the SOX proteins in this tissue. Here, we identified SOX9 in a SOX expression screening in the mouse fetal intestine. We report that the SOX9 protein is expressed in the intestinal epithelium in a pattern characteristic of WNT targets. We provide in vitro and in vivo evidence that a bipartite β-catenin/TCF4 transcription factor, the effector of the WNT signaling pathway, is required for SOX9 expression in epithelial cells. Finally, in colon epithelium-derived cells, SOX9 transcriptionally represses the CDX2 and MUC2 genes, normally expressed in the mature villus cells of the intestinal epithelium, and may therefore contribute to the WNT-dependent maintenance of a progenitor cell phenotype. 4
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study