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Author Wolf, Paul L. ♦ Muehil, Elisabeth Von Der ♦ Praisler, Karen
Source CiteSeerX
Content type Text
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Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Acid Phosphatase ♦ Serratia Organism ♦ Rapid Identification ♦ Bacterial Alkaline Phosphatase ♦ Alkaline Phosphatase Production ♦ Alkaline Phosphatase ♦ Coagulase-positive Staphylococcus ♦ Certain Hospitalized Patient ♦ Mannitol Fermentation ♦ Leucine Aminopeptidase ♦ Rapid Specific Method ♦ Precise Enzyme Lo-calization ♦ Bacterial Organism ♦ Previous Study ♦ Clinical Laboratory ♦ Leukocyte Al-kaline Phosphatase ♦ Excellent Correlation ♦ Histochemical Demon-stration ♦ Insol-uble Product ♦ Blue Color ♦ Specific Test ♦ Indigogenic Principle ♦ Life-threatening Complication ♦ Tissue Section ♦ Indolyl Phosphate ♦ Dnaase Activity ♦ Clinical Laborato-ries ♦ Coagulase-negative Staphylococcus ♦ Endo-and Exonuclease
Abstract This investigation concerns identification of alkaline phosphatase production by bacterial organisms, as detected by a blue color resulting from conversion of indolyl phosphate to indigo. Coagulase-positive Staphylococcus produced alkaline phosphatase; coagulase-negative Staphylococcus did not. Serratia did not produce alkaline phosphatase; those Entero-bacteriaceae we tested did. Thus, this test rapidly differentiates these organisms, diminishing the time for identification of Serratia in the clinical laboratory by 48 h. Identification of Serratia should not be ig-nored, because it is a life-threatening complication for certain hospitalized patients. Previously, we have described application of the indigogenic principle to the histochemical demon-stration of leucine aminopeptidase (EC 3.4.1.2) (1), 3-galactosidase (EC 3.2.1.23) (2), N-acetyl-13-glucosaminidase (EC 3.2.1.30) (3), alkaline and acid phosphatase (EC 3.1.3.1 and EC 3.1.3.2) (4), endo-and exonucleases (phosphodiesterases) (EC 3.1.4.5 and EC 3.1.4.6) (5), and serum (6) and leukocyte al-kaline phosphatase (EC 3.1.3.1) (7). The indolyl substrates offer the advantage of precise enzyme lo-calization with very little or no diffusion of the insol-uble products in tissue sections. Deoxyribonuclease (DNAase) activity, mannitol fermentation, and production of coagulase and pig-ment are indexes to pathogenicity of Staphylococci; measurement of DNAase activity may be superior to the other criteria (8). In this study we investigated a simple, specific test for detection of alkaline phosphatase production by Staphylococci. Our previous study of Staphylococci indicated excellent correlation of production of acid phosphatase and production of coagulase (9). The test for production of acid phosphatase was useful to differentiate Serratia from Enterobacteriaceae (10). However, testing for this enzyme in clinical laborato-ries has not been popular because of the lack of a rapid specific method.
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Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study