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Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Reduced-size Str Miniplex Primer Set ♦ Developmental Validation ♦ Validation Study ♦ Miniplex Set ♦ Dna Analysis Method ♦ Scientific Working Group ♦ Sensitive Tool ♦ Allele Consistency ♦ Codis Locus ♦ Pcr Amplification Condi-tions ♦ Standard Multiplex Kit ♦ Degraded Dna ♦ Forensic Sample ♦ Low Level Dna ♦ Miniplex Str Amplification Procedure ♦ Peak Balance ♦ New Set ♦ Developmental Validation Study ♦ Input Level ♦ Peak Intensity
Abstract ABSTRACT: This paper describes a developmental validation study of three Miniplex sets covering 12 of the 13 CODIS loci. As these new sets will be used for the analysis of degraded and low level DNA, the validation studies were performed using 100–125 pg of DNA, the lowest input level at which peak balance, peak intensity, and allele consistency were stable. To demonstrate the applicability of the Miniplex sets to forensic case-work, these validation studies were completed in accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM). A range of tests were performed including studies of concordance with standard multiplex kits, sensitivity and reproducibility, and PCR amplification condi-tions. Additionally, studies of mixtures, nonhuman and environmentally degraded DNA, and simulated forensic samples were performed. Our results demonstrate that Miniplex STR amplification procedures are a robust and sensitive tool for the analysis of degraded DNA.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article