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Author Recchia, Gustavo Henrique ♦ Gregorio, Danielle ♦ Caldas, Gomes ♦ Luiza, Ana ♦ Beraldo, Ahern ♦ Silva, Márcio José Da ♦ Tsai, Siu Mui
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Phaseolus Vulgaris ♦ Common Bean ♦ Tolerant Genotype ♦ Transcriptional Analysis ♦ Drought-induced Gene ♦ Expression Pattern ♦ Biological Process ♦ Low Technology Cultivation System ♦ Data Mining ♦ Ssh Library ♦ Drought Stress ♦ Transcription Factor ♦ Water Deficit ♦ Drought Response ♦ Sequence Tag ♦ Genotype Tolerant ♦ Suppressive Subtractive Hybridization ♦ Time-course Experiment ♦ Valid Read ♦ Rt-qpcr Experiment ♦ Genotype Carioca ♦ Proline-rich Protein ♦ Carbohydrate Metabolism
Abstract Abstract: In Brazil, common bean (Phaseolus vulgaris L.) productivity is severely affected by drought stress due to low technology cultivation systems. Our purpose was to identify differentially expressed genes in roots of a genotype tolerant to water deficit (BAT 477) when submitted to an interruption of irrigation during its development. A SSH library was constructed taking as “driver ” the genotype Carioca 80SH (susceptible to drought). After clustering and data mining, 1572 valid reads were obtained, resulting in 1120 ESTs (expressed sequence tags). We found sequences for transcription factors, carbohydrates metabolism, proline-rich proteins, aquaporins, chaperones and ubiquitins, all of them organized according to their biological processes. Our suppressive subtractive hybridization (SSH) library was validated through RT-qPCR experiment by assessing the expression patterns of 10 selected genes in both genotypes under stressed and control conditions. Finally, the expression patterns of 31 ESTs, putatively related to drought responses, were analyzed in a time-course experiment. Our results confirmed that such genes are more expressed in the tolerant genotype during stress; however, they are notInt. J. Mol. Sci. 2013, 14 7156
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2013-01-01