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Author Colucci, Silvia ♦ Brunetti, Giacomina ♦ Oranger, Angela ♦ Mori, Giorgio ♦ Grano, Zallone Maria
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Osteoclast Apoptosis ♦ Trail Treatment ♦ Apoptotic Process ♦ Critical Regulatory Factor ♦ Oc Differentiation ♦ Considerable Attention ♦ Novel Strategy ♦ Oc Death ♦ Apoptotic Oc Number ♦ Death Receptor ♦ Neoplastic Cell ♦ Trail Receptor ♦ Many Normal Cell Trail ♦ Bone Remodelling ♦ Oc Apoptosis ♦ Anti-dr5 Neutralizing Antibody ♦ Different Trail Receptor Expression Pattern ♦ Bid Basic Applied Myology ♦ Tnf-related Apoptosis-inducing Ligand ♦ Unique Property ♦ Drug Development ♦ Key Word ♦ First Time ♦ Dna Ladder ♦ Malignant Osteolysis ♦ Cell Viability
Abstract Control of osteoclast (OC) apoptosis has been recognized as a critical regulatory factor in bone remodelling, and alteration in OC death and/or survival has been shown to contribute to the pathogenesis of osteoporosis or malignant osteolysis. TNF-related apoptosis-inducing ligand (TRAIL) has received considerable attention as a molecule showing the unique property to induce apoptosis in a variety of neoplastic cells, however, in many normal cells TRAIL can activate the apoptotic process. In this paper, we demonstrate for the first time a different TRAIL receptor expression pattern during OC differentiation. Moreover, we show that TRAIL treatment causes an increased apoptotic OC number, caspase 8 and 3 activation and the formation of the DNA ladder. The apoptotic process is mediated by death receptor 5 (DR5) as demonstrated by the increase in its expression following TRAIL treatment and by the rescue of the cell viability in OCs treated simultaneously with TRAIL and anti-DR5 neutralizing antibody. Elucidation of the mechanisms controlling OC apoptosis may reveal novel strategies for drug development. Key Words: TRAIL, TRAIL receptors, osteoclasts, apoptosis, caspase-8, Bid Basic Applied Myology 19 (2&3): 131-138, 2009
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study