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Author Forouzanfar, Mohsen ♦ Hosseini, Sayyed Mortaza ♦ Hajian, Mehdi ♦ Molavi, Fariba ♦ Abedi, Parvaneh ♦ Hossein, Mohammad ♦ Esfahani, Nasr
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Bovine Embryo ♦ Vero Cell ♦ Co-culture System ♦ Icm Te ♦ Differential Effect ♦ Presumptive Zygote ♦ Significant Difference ♦ Undergo Fertilization ♦ Total Cell Number ♦ Menezo B2 ♦ Tissue Culture Medium ♦ B2 Culture Medium ♦ Inner Cell Mass ♦ Culture Medium ♦ Bovine Oocyte ♦ Blastocyst Stage ♦ 8-16 Cell ♦ Tropho Ectoderm ♦ Blastocyst Underwent Differential Staining ♦ Vitro Developmental Quantity
Abstract Background: This study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions, tissue culture medium 199 (TCM199)–vero cells and Menezo B2 (B2)-vero cells, for the in vitro developmental quantity and quality of bovine embryos. Materials and Methods: Bovine oocytes were allowed to mature and subsequently undergo fertilization in vitro. Their presumptive zygotes were cultured in either TCM199 or B2 culture media in conjunction with vero cells for up to nine days. The culture media were refreshed every two days and the proportion of embryos that cleaved and further developed to the morula and blastocyst (early, expand and hatched) stages were recorded. Hatched blastocysts underwent differential staining in order to determine the numbers of inner cell mass (ICM) and tropho ectoderm (TE) and total cell number (TCN). Results: Of the two groups, no significant difference was seen between the proportions of the presumptive zygotes cleaved, those which developed to 8-16 cells, morula and reached days 7or 8 blastocyst stage or hatched. However, the values for TCN and TE of the TCM199-
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Publisher Date 2009-01-01