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Author Ijpnl, Coden ♦ Priyanka, J. ♦ Kumar, P. Anil
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Mobile Phase ♦ Recovery Study ♦ Flow Rate ♦ Degassed Mixture ♦ Thermo Hypersil Bd ♦ Run Time ♦ Uv Detection ♦ Known Quantity ♦ Specific Rp-hplc 1-5 Method ♦ Test Solution ♦ Precision Rsd ♦ Routine Quality Control ♦ Standard Drug ♦ Concentration Range
Abstract A rapid, sensitive and specific RP-HPLC [1-5] method involving UV detection was developed and validated for determination and quantification of Lamivudine and Zidovudine. Chromatography was carried out on Thermo Hypersil BDS, C18,(150 x 4..6 mm,5�) column using filtered and degassed mixture of Buffer: Methanol: Acetonitrile (70:5:25) as mobile phase at a flow rate of 0.8ml/min and effluent was monitored at 267nm. The method was validated in terms of linearity, precision, accuracy, robustness and specificity, limit of quantification and limit of detection. The assay was linear over the concentration range of Lamivudine and Zidovudine was 37.5µg- 225µg/ml and 75µg to 450µg/ml respectively. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analyzed test solution and was found to be 99.50%-100.7 % and 99.9%-100.7 % within precision RSD of 1.30 and 0.61 for Lamivudine and Zidovudine respectively. The method does require only 10 minutes as run time for analysis which prove the adoptability of the method for the routine quality control of the drug.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study