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Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Novel Rat Gene ♦ Putative Membrane Protein ♦ N-myristoylation Site ♦ Cdna Subtraction ♦ Latex Bead ♦ Multiple Possible Phosphorylation Site ♦ Cdna Cloning ♦ Normal Liver ♦ Tissue-specific Gene Expression ♦ Tissue-specific Putative Membrane Protein ♦ Northern Blot Analysis ♦ Untranslated Region ♦ Key Word ♦ Rat Liver ♦ Db83 Gene ♦ Race Technique ♦ Hydrophobic Irons-membrane Domain ♦ Novel Cdna ♦ Par-tial Cdna Insert ♦ Previous Work ♦ Partial Cdna Sequence
Abstract Using a partial cDNA sequence and a 5'-RACE technique, we isolated a novel cDNA from rat liver referred to as DB83. DB83 had four hydrophobic irons-membrane domains and one N-myristoylation site as well as multiple possible phosphorylation sites. The db83 gene was highly expressed in the liver and significantly in brain, ' lungs and kidneys. We suggest that DB83 is a tissue-specific putative membrane protein. Key words: cDNA cloning; membrane protein; tissue-specific gene expression We have studied genes which are expressed abundantly in the liver. In a previous work, we obtained 13 par-tial cDNA inserts from rat liver by cDNA subtraction between normal liver and hepatoma using oligo(dT)-immobilized Latex beads.1 One of those inserts, named DB83, was about 500 bp in length. Afterward, this re-gion was mapped in the DB83 3'-untranslated region from 1308 to 1795 (Fig. 1). Northern blot analysis us-
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Publisher Date 1998-01-01