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Author Syiwester, Andrew ♦ Shutt, Damon ♦ Wesseis, Deborah ♦ Stapleton, Jack ♦ Stites, T. Jan ♦ Kennedy, Ronald C. ♦ Soli, David A.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Motility Cycle ♦ Hiv-induced Cell Syncytium ♦ Rat Tail Collagen ♦ Cd4-enriched Pbmcs ♦ Dictyostelium Ameba ♦ Pbmc Syn-cytia ♦ Substrate Plastic ♦ Human Polymorphonuclear Leu-kocytes ♦ Animal Cell ♦ Average Cell Velocity ♦ Single Cell ♦ Cyclical Fashion ♦ Immortal Suptb Cell Line ♦ Hiv-induced Suptl ♦ Bovine Aortic En-dothelium ♦ Peripheral Blood Mononuclear ♦ Cd4-positive Cell ♦ Ameboid Cell ♦ Human Immuno-deficiency Virus ♦ Cell Size ♦ Supti Cell ♦ Computer-assisted Motion Analysis ♦ Single-cell Progeni-tors ♦ Cell Type ♦ Human Lymphocyte
Abstract Abstract: Ameboid cells ranging in complexity from Dictyostelium amebas to human polymorphonuclear leu-kocytes (PMNs) translocate in a cyclical fashion. Using computer-assisted motion analysis, we have analyzed the motility of human lymphocytes of the immortal SupTb cell line and of a peripheral blood mononuclear cell popu-lation highly enriched for CD4-positive cells (CD4-enriched PBMCs) on four substrates- plastic, dehydrated rat tail collagen, hydrated rat tail collagen, and bovine aortic en-dothelium. In addition, we have analyzed the motility on these substrates of syncytia induced by human immuno-deficiency virus (HIV) in cultures of both cell types. It is demonstrated that both SupTi cells and CD4-enriched PBMCs exhibit a motility cycle with a period of 1.6 mm that is independent of substrate, independent of average cell velocity, and similar to the periods of transbocating Dictyostelium amebas and PMNs. More surprisingly, it is demonstrated that HIV-induced SupTl and PBMC syn-cytia with volumes 10 to 100 times those of single cells ex-hibit the same motility cycle as their single-cell progeni-tors. These observations support the generality of the motility cycle in animal cells and, for the first time, demonstrate that the cycle is independent of cell size. j
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article