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Author Stroschein-Stevenson, Shannon L. ♦ Foley, Edan ♦ O.’farrell, Patrick H. ♦ Johnson, Er D.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Efficient Phagocytosis ♦ Plo Biol ♦ Drosophila Gene Product ♦ Subsequent Phagocytosis ♦ Conserved Aspect ♦ Certain Class ♦ Actin Cytoskeleton Regulation ♦ Gene Product ♦ Staphylococcus Aureus ♦ Model System ♦ Rnai Library ♦ Drosophila Thioester Protein ♦ Diverse Biological Process ♦ Drosophila Melanogaster S2 Cell ♦ Transcriptional Regulation ♦ Initial Gene ♦ Major Fungal Pathogen ♦ Vesicle Transport ♦ Macroglobulin Complement ♦ Escherichia Coli ♦ Secondary Screen ♦ Latex Bead ♦ Innate Immunity ♦ Albicans Phagocytosis ♦ Fly Protein Distinguishes Different Pathogen ♦ Fly Gene ♦ Several Gene
Abstract Phagocytosis is a highly conserved aspect of innate immunity. We used Drosophila melanogaster S2 cells as a model system to study the phagocytosis of Candida albicans, the major fungal pathogen of humans, by screening an RNAi library representing 7,216 fly genes conserved among metazoans. After rescreening the initial genes identified and eliminating certain classes of housekeeping genes, we identified 184 genes required for efficient phagocytosis of C. albicans. Diverse biological processes are represented, with actin cytoskeleton regulation, vesicle transport, signaling, and transcriptional regulation being prominent. Secondary screens using Escherichia coli and latex beads revealed several genes specific for C. albicans phagocytosis. Characterization of one of those gene products, Macroglobulin complement related (Mcr), shows that it is secreted, that it binds specifically to the surface of C. albicans, and that it promotes its subsequent phagocytosis. Mcr is closely related to the four Drosophila thioester proteins (Teps), and we show that TepII is required for efficient phagocytosis of E. coli (but not C. albicans or Staphylococcus aureus) and that TepIII is required for the efficient phagocytosis of S. aureus (but not C. albicans or E. coli). Thus, this family of fly proteins distinguishes different pathogens for subsequent phagocytosis.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Publisher Date 2006-01-01