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Author Gerrits, Margot I. ♦ Jssen, Jos H. H. Thi.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Special Attention ♦ Movmol Creatinine ♦ Bone Resorption ♦ Chemical Stability ♦ Biochem-ical Marker ♦ Stored Urine ♦ Signifi-cant Change ♦ 10-20 Year ♦ Free Dpyr ♦ Free Pyr ♦ Urinary Excretion ♦ Hplc Method ♦ Systematic Investigation ♦ Present Study ♦ Long-term Stability ♦ Excretion Pattern ♦ Pyridinium Crosslinks
Abstract Urinary excretion of the pyridinium crosslinks pyridinoline (Pyr) and deoxypyridinoline (Dpyr) is used as a biochem-ical marker of bone resorption. The present study was undertaken to determine the long-term stability of these compounds in stored urine, using the HPLC method. Systematic investigation of their chemical stability in urine demonstrated that both the free and conjugated forms of Pyr and Dpyr are extremely stable: No signifi-cant changes were observed after 6 weeks at-20#{176}C storage (e.g., free Pyr 9.6 ± 1.2 moVmol creatinine (before) and 10.6 ± 3.2 (after); free Dpyr 2.3 ± 0.2 moVmol creatinine (before) and 2.5 ± 1.2 (after)). These results predict stability of unnes stored for 10-20 years at-20#{176}Cin the dark. Also, freezing and thawing as many as 10 times had no effect on the concentrations of the crosslinks. Study of the stability of the excretion pattern
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study