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Author Didenko, Vladimir V. ♦ Ngo, Hop ♦ Baskin, David S.
Source CiteSeerX
Content type Text
File Format PDF
Language English
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Blunt-ended Break ♦ Apoptotic Dna Degrada-tion ♦ Necrotic Dna Degradation ♦ Early Ne-crosis ♦ Cell Culture Model ♦ Dna Break ♦ Rat Thymus ♦ Early Necrotic Cell ♦ Blunt End ♦ Cell Type ♦ Blunt-ended Dna Break ♦ Early Necrosis ♦ Necrotic Tissue ♦ Cell Death ♦ Cell Culture ♦ Situ Ligation ♦ Double-strand Dna ♦ Jurkat Cell ♦ Selective Detection
Description The structure of DNA breaks in early necrosis was analyzed and compared with apoptotic DNA degrada-tion using in vivo and cell culture models. Early ne-crosis (1 hour after cell death) was produced in vivo by the freezing-thawing of rat thymus and in cell culture of Jurkat cells. Apoptosis was induced in the same cell types using dexamethasone for thymus and staurosporine for Jurkat cells. Selective detection of double-strand DNA breaks with blunt ends was per-formed by in situ ligation. Blunt-ended breaks bear-ing 5 phosphates were detected in apoptotic but not in early necrotic cells. Pretreatment of apoptotic and necrotic tissue with Klenow enzyme with or without added dNTPs reduced all 3 or 5 overhangs to blunt ends. Subsequent in situ ligation with blunt-ended
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2003-01-01
Publisher Institution http://mic.sgmjournals.org 41 Metronidazole-induced apoptosis in Blastocystis