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Author Isolated, Lipoprotein ♦ Pennathur, Subramaniam ♦ Heinecke, Jay W.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Low Density ♦ Protein Oxidation ♦ Hydroxyl Radical ♦ Mass Spectrometric Quantification ♦ Tyrosyl Radical ♦ Metal Ion ♦ Active Metal Ion ♦ Understood Pathway ♦ Potential Mech-anisms ♦ Tyrosyl Radical Form ♦ Quantitative Method ♦ Physio-logically Relevant Pathway ♦ Low Density Lipopro-tein ♦ Stable Isotope Dilution ♦ Dityrosine Cross-link ♦ Lipoprotein Oxidation ♦ Oxidative Dam-age ♦ Reactive Hydroxyl Radical Oxidizes
Abstract Lipoprotein oxidation has been implicated in the pathogenesis of atherosclerosis. However, the physio-logically relevant pathways mediating oxidative dam-age have not yet been identified. Three potential mech-anisms are tyrosyl radical, hydroxyl radical, and redox active metal ions. Tyrosyl radical forms o,o*-dityrosine cross-links in proteins. The highly reactive hydroxyl radical oxidizes phenylalanine residues to o-tyrosine and m-tyrosine. Metal ions oxidize low density lipopro-tein (LDL) by poorly understood pathways. To explore the involvement of tyrosyl radical, hydroxyl radical, and metal ions in atherosclerosis, we developed a highly sensitive and quantitative method for measuring levels of o,o*-dityrosine, o-tyrosine, andm-tyrosine in proteins, lipoproteins, and tissue, using stable isotope dilution
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study