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Author Coulombe, Pierre A. ♦ Wawersik, Matthew ♦ Paladini, Rudolph D. ♦ Noensie, Erick
Source CiteSeerX
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Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Biochemical Basis ♦ Functional Implication ♦ Various Type Ii Keratin Partner ♦ Nonepithelial Cell Line ♦ Adhesion Property ♦ Injury-undergo Major Cytoarchitectural Alteration ♦ Type Ii Keratin Partner ♦ Intrinsic Property ♦ Direct Role ♦ Epidermal Keratinocytes ♦ Tetramer Subunit Predomi-nates ♦ Surface Morphol-ogy ♦ Wound Edge ♦ K16 Form Unstable Heterotetramer Subunit ♦ Soluble Pool ♦ Dynamic Parti-this Paper ♦ Tetramer-forming Property ♦ Related Type ♦ Particular Interest ♦ Keratinocyte Activation ♦ Protein Expression ♦ Wound-edge Keratinocytes ♦ Intracellular Organization
Abstract (K16 and K17) are intermediate-filament (IF) proteins that are induced in wound-edge keratinocytes as early as 4-6 h after injury to skin, either human or mouse. This induction occurs at the expense of the keratin proteins that are normally expressed in differentiating epidermal keratinocytes. Correlated with these changes in protein expression, keratinocytes-for 24 h following injury-undergo major cytoarchitectural alterations that affect their shape, intracellular organization, surface morphol-ogy, and adhesion properties. We recently proposed that the intrinsic properties of K16 are compatible with a direct role in “keratinocyte activation ” at the wound edge (Pal-adini et al., 1996). Unlike K14, a related type I keratin that is constitutively expressed in epidermis, we found that K16 forms unstable heterotetramer subunits that po-lymerize into shorter filaments when paired with a variety of type II keratin partners (e.g., K5, K6b, K8). Such prop-erties are of particular interest because it has been shown in a number of studies that the tetramer subunit predomi-nates in the soluble pool of IF subunits in epithelial and nonepithelial cell lines in culture. The tetramer-forming properties of K16 may thus influence its dynamic parti-This paper was originally presented at a workshop titled The Cytoskel-
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