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Author Oldiield, S. ♦ Hirasawa, T. ♦ Roy, P.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Bluetongue Virus ♦ Outer Capsid Protein ♦ Sequence Conservation ♦ Contrasting Feature ♦ Outer Capsid Protein Vp2 ♦ Encoded Protein ♦ Genome Consists ♦ Non-structural Protein ♦ Dsrna Segment ♦ Minor Protein ♦ Core Particle ♦ Knudson Shope ♦ South African ♦ Btv Serotype ♦ Infected Cell ♦ Important Pa Hogen ♦ Single Protein ♦ Different Serotypes ♦ Amino Acid Sequence ♦ Major Serotype-specific Antigen ♦ Icosahedral Symmetry ♦ Rna Segment ♦ Btv Serotypes ♦ Btv United State Serotypes ♦ Many Part ♦ Complete Nucleotide Sequence ♦ Diffuse Outer Layer ♦ Major Protein ♦ Dsrna Virus ♦ United State ♦ Geographi-cal Separation ♦ Cdna Clone
Abstract The complete nucleotide sequence of a cDNA clone representing the segment 5 RNA of bluetongue virus (BTV) United States erotype 13 was determined. The comparison of the predicted amino acid sequence of the encoded protein (VP5) with the sequences ofVP5 from two BTV United States serotypes, 10 and 2, and two isolates of BTV serotype I (Australian and South African), revealed that the protein is highly conserved among the different serotypes despite their geographi-cal separation. Bluetongue virus (BTV) is a dsRNA virus that is an economically important pa hogen of sheep and cattle in many parts of the world. The genome consists of 10 dsRNA segments (Verwoerd et al., 1970), each of which encodes a single protein (Mertens et al., 1984). The virion consists of a core of icosahedral symmetry composed of two major proteins (VP3 and VP7) enclosing three minor proteins (VP 1,VP4 and VP6), together with the 10 RNA segments (Verwoerd et al., 1972). This core particle is surrounded by a diffuse outer layer of two further proteins (VP2 and VP5). Three non-structural proteins are also synthesized in infected cells but these are not incorporated into the virion (Huismans, 1979). At least 24 BTV serotypes have been identified (Gorman et al., 1983; Knudson & Shope, 1985) and VP2 has been shown to be the major serotype-specific antigen
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study