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Author Piyali, M.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Cell Wall ♦ Electron Microscopic Study ♦ Melanin Layer ♦ Transmission Electron Microscopy ♦ Melanin Lacking ♦ Cryptococcus Neoformans ♦ Life-threatening Infection ♦ Cell Wall Size ♦ Albino Strain ♦ Dual Cryptococcal Infection ♦ Study Tem ♦ Melanotic Strain ♦ Major Virulence Factor ♦ Central Nervous System ♦ Important Opportunistic Fungal Pathogen ♦ Cell Wall Area
Abstract Cryptococcus neoformans is an important opportunistic fungal pathogen that causes life-threatening infection of the central nervous system. A major virulence factor for C. neoformans is the production of melanin in the cell wall. Using transmission electron microscopy, we studied the cell walls of three pairs of isolates obtained from patients with dual cryptococcal infections, where a melanotic and an albino strain were isolated from the CSF of each patient. Transmission Electron Microscopy revealed that the albino strains lacked a melanin layer whereas a melanin layer was associated with the cell wall of the melanotic strains, comprising approximately 75 % of the cell wall area. The cell wall size of the melanin producing cells was approximately double the size the albino isolates ’ cell walls (p value ≤ 0.003). In this study TEM revealed that the differences in the ultrastructure of the melanin lacking and melanin producing isolates were associated to the cell wall and the melanin layer.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study