Thumbnail
Access Restriction
Open

Author Quinkler, M. ♦ Troeger, H. ♦ Eigendorff, E. ♦ Maser-Gluth, C. ♦ Stiglic, A. ♦ Oelkers, W. ♦ Bähr, V. ♦ Diederich, S.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Stress Adaptation ♦ Guinea Pig ♦ 11-hydroxysteroid Dehydrogenase Type ♦ Glucocorticoid Receptor ♦ 3h-labelled Cortisol ♦ Intracellular Cortisol Level ♦ Vice Versa ♦ 11-hsd Type ♦ Vitro Effect ♦ Guinea Pig Liver ♦ Untreated Guinea Pig ♦ High Cortisol Concentration ♦ Inactive Metabolite Cortisone ♦ Mineralocorticoid Receptor ♦ 11-hsd Activity ♦ 11-hydroxysteroid Dehydrogenases ♦ Natural Gluco-corticoids ♦ Convert Cortisol ♦ Tissue Slice
Abstract (M Quinkler and H Troeger contributed equally to this work) The 11-hydroxysteroid dehydrogenases (11-HSDs) convert cortisol to its inactive metabolite cortisone and vice versa. 11-HSD type 1 (11-HSD-1) functions as a reductase in vivo, regulating intracellular cortisol levels and its access to the glucocorticoid receptor. In contrast, 11-HSD-2 only mediates oxidation of natural gluco-corticoids, and protects the mineralocorticoid receptor from high cortisol concentrations. We investigated the in vivo and in vitro effects of ACTH on the recently characterized 11-HSDs in guinea pig liver and kidney. Tissue slices of untreated guinea pigs were incubated with 3H-labelled cortisol or cortisone and ACTH1–24 (1010 and 109 mol/l). The 11-HSD activities in
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study