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Author P. -Glycoprotein, I. ♦ Zhang, Lihong ♦ Jacobsson, Karin ♦ Strom, Katrin ♦ Lindberg, T. Martin ♦ Frykberg, Lars
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Protein Sbi ♦ Staphylococcus Aureus ♦ Second Igg-binding Protein ♦ Later Data ♦ Amino Acid ♦ Gpi-binding Activity ♦ Immobilized Protein Sbi ♦ Shotgun Phage Display ♦ Serum Component ♦ Igg-binding Domain ♦ Gpi-binding Domain ♦ Staphy Ococcus Aureus ♦ Staphylococcal Cell Surface
Abstract The existence of a second IgG-binding protein, protein Sbi, in Staphy/ococcus aureus has been reported previously. Later data indicated that protein Sbi also bound another serum component. This component has now been affinity-purified on immobilized protein Sbi and identified as P,-glycoprotein I (/?,-GPI), also known as apolipoprotein H. The minimal P,-GPI-binding domain was identified by shotgun phage display and the binding was shown to be mediated by a region of 57 amino acids, clearly separated from the IgG-binding domain. It is also shown that protein Sbi, and thus the P,-GPI-binding activity, is expressed on the staphylococcal cell surface at levels varying between strains.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study