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Author Sabbaghian, E. S. ♦ Roostaazad, R.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Soy Flour ♦ Substrate Particle Size ♦ Lipase Production ♦ Trace Element ♦ Lipase Productivity ♦ Semi-solid Medium ♦ Optimum Concentration ♦ Different Factor ♦ Optimum Condition ♦ Cell Cultivation ♦ Taguchi Method ♦ Mesh Size Cut ♦ Abstract Pseudomonas ♦ Medium Composition ♦ Maximum Lipase Activity ♦ Optimum Value ♦ Unit Ml
Abstract Abstract – Pseudomonas aeoroginosa B-3556 was grown on soy flour based semi-solid media for lipase production. The effect of different factors including medium composition, pH, temperature, mixing intensity and substrate particle size on lipase productivity was analyzed using the Taguchi method in three steps. Based on these analyses the optimum concentrations of soy flour, (NH4)2HPO4, K2HPO4 and MgS04.7H2O were found to be 3%, 3%, 0.5 % and 0.21 % respectively. Furthermore, optimum values for temperature, pH and the shaking speed were determined as 30 °C, 7.1 and 150 rpm. As for the substrate particle size, the best result was achieved when the soy flour had a mesh size cut of 60-70. The influence of the trace elements on lipase production was studied and it was shown that their addition to the medium was not necessary. Maximum lipase activity under optimum conditions was obtained as 44.8 unit/ml after 48 hrs of cell cultivation.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study