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Author Burke, Valerie ♦ Robinson, Jennifer ♦ Berry, R. J. ♦ Gracey, M. ♦ Xxviii, Plate
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Gastroenterological Research Unit ♦ Per Th ♦ Princess Margaret Child ♦ Medical Research Foundat Ion ♦ Enterotoxigenic Strain ♦ Yeast Extract ♦ Epidemiological Study ♦ Water Bath ♦ Ratio Intestinal Weight ♦ Aeromonas Hydrophila ♦ Tryptone Soya Broth ♦ Toxin Testing ♦ Environmental Incubator ♦ Suckling-mouse Assay ♦ Body Weight ♦ Scoring System ♦ Standard Condi-tions
Abstract SUMMARY. The suckling-mouse assay was reliable for detecting enterotoxigenic strains of Aeromonas hydrophila when standard condi-tions for growth and toxin testing were used. Enterotoxins were produced by bacteria grown in tryptone soya broth supplemented with yeast extract and aerated by shaking in an environmental incubator or water bath. When culture supernates together with dye were adminis-tered intragastrically to mice less than 6 days old, the presence of enterotoxin was assessed on the basis of a scoring system that incorporated the ratio intestinal weight: remaining body weight, and production of diarrhoea. This method should facilitate the detection of enterotoxigenic strains of Aeromonas in epidemiological studies.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study