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Author Mercer, Andrew A. ♦ Mitra, Alok K.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Electron Microscopy ♦ Large Icosahedral Dna Virus ♦ Immature Poxvirus Particle ♦ Orf Virus ♦ Capsid Protein ♦ Putative Scaffolding Protein ♦ Prototype Parapoxvirus ♦ Viral Genome Code ♦ Three-dimensional Structure ♦ Two-dimensional Crystal ♦ Good Agreement ♦ Contagious Ecthyma ♦ Essential Protein ♦ Previous Study ♦ Genome Sequence ♦ Orfv075 Belongs ♦ Protein Orfv075 ♦ Central Region ♦ Vaccinia Virus Morphogenesis ♦ Many Double-stranded Dna Icosahedral Virus ♦ Major Capsid Protein ♦ Double-barreled Capsid Protein Family ♦ Vaccinia Virus Immature Virion ♦ Orfv075 Show ♦ Rifampin Resistance Gene Product ♦ Mandatory Intermediate ♦ Vertebrate Poxvirus ♦ Molecular Envelope ♦ Viral Proliferation ♦ Distant Homologue ♦ Nonicosahedral Poxvirus ♦ Viral Maturation ♦ Paramecium Bursaria Chlorella Virus Type ♦ Tripod-like Main Body ♦ Structural Analysis ♦ Recombinant Orfv075
Abstract Orf virus, the prototype parapoxvirus, is responsible for contagious ecthyma in sheep and goats. The central region of the viral genome codes for proteins highly conserved among vertebrate poxviruses and which are frequently essential for viral proliferation. Analysis of the recently published genome sequence of orf virus revealed that among such essential proteins, the protein orfv075 is an orthologue of D13, the rifampin resistance gene product critical for vaccinia virus morphogenesis. Previous studies showed that D13, arranged as “spicules, ” is necessary for the formation of vaccinia virus immature virions, a mandatory intermediate in viral maturation. We have determined the three-dimensional structure of recombinant orfv075 at 25-Å resolution by electron microscopy of two-dimensional crystals. orfv075 organizes as trimers with a tripod-like main body and a propeller-like smaller domain. The molecular envelope of orfv075 shows unexpectedly good agreement to that of a distant homologue, VP54, the major capsid protein of Paramecium bursaria Chlorella virus type 1. Our structural analysis suggests that orfv075 belongs in the double-barreled capsid protein family found in many double-stranded DNA icosahedral viruses and supports the hypothesis that the nonicosahedral poxviruses and the large icosahedral DNA viruses are evolutionarily related. Orf virus (ORFV) is the prototype parapoxvirus, infecting sheep and goats worldwide. It is responsible for contagious
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Publisher Date 2007-01-01