Thumbnail
Access Restriction
Open

Author Pcc, Synechocystis ♦ Komenda, Josef ♦ Benešová, Julie ♦ Tichý, Martin
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Cellular Molecular Biology Letter ♦ Rc47 Complex ♦ Blue Native Electrophoresis ♦ Wild Type ♦ Western Blot ♦ Cyanobacterium Synechocystis Pcc ♦ Rc Complex Consisting ♦ Radioactive Labeling ♦ Small Photosystem Ii ♦ Original Psbh Gene ♦ Psii Assembly ♦ Psbh Protein ♦ Psbh Gene ♦ Complex Corresponding ♦ Labeled Psbh Protein ♦ Assembly Process ♦ Psba2 Promotor ♦ Core Complex ♦ Active Psii Complex ♦ Double Mutant ♦ Assemble Rc Complex ♦ Psii Core Complex ♦ Unassembled Cp47
Abstract The PsbH protein, a small Photosystem II (PSII) subunit, has been previously identified in the cyanobacterium Synechocystis PCC 6803 as a component of the PSII core complex (RCC), the core complex lacking CP43 (RC47) but not the RC complex consisting of D1, D2 and cytochrome b-559 [1]. To follow the insertion of the protein into PSII during the assembly process, we constructed a mutant in which the original psbH gene was deleted and a modified psbH gene with the 6 x His tag at the N-terminus was inserted under the psbA2 promotor. Phenotype of this mutant was indistinguishable from the wild type indicating presence of fully active PSII complexes. Analysis of the PSII assembly in the mutant using blue native electrophoresis in combination with radioactive labeling and Western blot confirmed that the protein is incorporated into PSII during the step when CP47 is attached to the RC complex forming the RC47 complex. However, the labeled PsbH protein was found not only RCC and RC47 complex but also in the smaller complex corresponding to the unassembled CP47. In the double mutant unable to assemble RC complex due to deletion of
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study