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Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Benchmark Amplified Fragment-length Poly ♦ Primer Pair ♦ Labeled Primer ♦ Germplasm Characterization ♦ Capillary Array System ♦ High Band Yield ♦ Restriction-based Molecular Marker ♦ Diverse Application ♦ Random-amplified Polymorphic Dna ♦ Random Location ♦ Restriction Fragment-length Polymorphism ♦ Large Number ♦ Priori Sequence Knowledge ♦ Popular Tech-nique ♦ Link-age Mapping ♦ Aflp-based Genetic Fingerprint ♦ Aflp Marker
Abstract morphism (AFLP) is a popular tech-nique that uses linkers to combine ele-ments from restriction fragment-length polymorphism (RFLP) and random-amplified polymorphic DNA (RAPD) methods to PCR amplify restriction-based molecular markers from random locations in the genome. AFLP markers are widely utilized because of repro-ducibility, high band yield per primer pair, and a priori sequence knowledge is not required (1). Automation using capillary array systems and fluores-cently labeled primers makes AFLP particularly appealing to researchers with large numbers of samples. Among the diverse applications for AFLP-based genetic fingerprints, it is most useful in breeding programs involving germplasm characterization and link-age mapping (2,3).
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study