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Author Hatch, Marguerite ♦ Bourke, E. ♦ Costello, J.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Serum Oxalate Determination ♦ New Enzymic Method ♦ Oxalate Determination ♦ Sensitive Simplified Enzymic Method ♦ Formate Dehydrogenase ♦ Subsequent Colorimetric Determination ♦ Deter-mining Serum Oxalate ♦ Nad-requiring Formate Dehydrogenase ♦ Healthy Volunteer ♦ Recently Knowles ♦ Nor-mal Value ♦ Several Recent Method ♦ Oxalate Decarboxylase ♦ Human Serum ♦ Routine Use ♦ Reproducible Result ♦ Serum Ultrafiltration ♦ Oxalate Decarboxyl-ase ♦ Mg Liter ♦ Enzymic Method ♦ Evolved Co2 ♦ Resultant Formate ♦ Mean Value
Abstract We describe a sensitive simplified enzymic method for serum oxalate determination. After serum ultrafiltration, precipitation, and extraction, oxalate is decarboxylated and the resultant formate is measured spectrophotometrically by using NAD-requiring formate dehydrogenase. There have been several recent methods for deter-mining serum oxalate in man (1-8), but reported nor-mal values have ranged from 0.3 to 14 mg/liter. Recently Knowles and Hodgkinson (6) reported an enzymic method for oxalate determination in human serum by use of oxalate decarboxylase (EC 4.1.1.2) with subsequent colorimetric determination of the evolved CO2. The method is specific, but the technique is rather too elaborate for routine use. Our method also involves use of oxalate decarboxyl-ase in a linked reaction with NAD+ requiring formate dehydrogenase (EC 1.2.1.2). Determinations are done spectrophotometrically, with reproducible results. We have recently applied the principle successfully for oxalate determinations in urine (9). In serum the mean value for data from 40 healthy volunteers was 1.82 mg/liter, the values for women being significantly higher than for men. Materials and Methods
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study