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Author Methyltransferases, Bacterial ♦ Tavassoli, Ali ♦ Shapiro, Lucy
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Borinic Ester ♦ Bacterial Cell Growth ♦ Caulobacter Crescentus Ccrm ♦ Minimum Inhibitory Concentration ♦ Current Antibiotic ♦ Essential Dna Methyltransferase ♦ Boron-containing Compound ♦ Broad Spectrum Antibacterial Activity ♦ Essential Bacterial Enzyme ♦ Specific Inhibitor ♦ Menaquinone Methyltransferase ♦ Low Microgram Ml Range ♦ Gram Negative R-proteobacteria ♦ Bacillus Subtilis ♦ New Biochemical Assay ♦ Continual Battle ♦ Gram Positive Bacteria ♦ New Antibacterial Agent ♦ Antibacterial Agent
Abstract As bacteria continue to develop resistance toward current antibiotics, we find ourselves in a continual battle to identify new antibacterial agents and targets. We report herein a class of boron-containing compounds termed borinic esters that have broad spectrum antibacterial activity with minimum inhibitory concentrations (MIC) in the low microgram/mL range. These compounds were identified by screening for inhibitors against Caulobacter crescentus CcrM, an essential DNA methyltransferase from Gram negative R-proteobacteria. In addition, we demonstrate that borinic esters inhibit menaquinone methyltransferase in Gram positive bacteria using a new biochemical assay for MenH from Bacillus subtilis. Our data demonstrate the potential for further development of borinic esters as antibacterial agents as well as leads to explore more specific inhibitors against two essential bacterial enzymes.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Publisher Date 2005-01-01