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Author Nurmemmedov, Elmar ♦ Castelnovo, Martin ♦ Medina, Elizabeth ♦ Catalano, Carlos Enrique ♦ Evilevitch, Alex
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Terminase Motor ♦ Packaging Limit ♦ Dna Length ♦ Packaging Strategy ♦ Dna Phage ♦ Upper Limit ♦ Building Phage ♦ Infectious Particle ♦ Progressive Build-up ♦ Biomolecular World ♦ Packaging Process ♦ Phage Production Rate ♦ Several Ten ♦ Active Machine ♦ Viral Genome ♦ Experimental Work ♦ Kilobase Pair ♦ Infectious Virion
Abstract The terminase motors of bacteriophages have been shown to be among the strongest active machines in the biomolecular world, being able to package several tens of kilobase pairs of viral genome into a capsid within minutes. Yet these motors are hindered at the end of the packaging process by the progressive build-up of a force resisting packaging associated with already packaged DNA. In this experimental work, we raise the issue of what sets the upper limit on the length of the genome that can be packaged by the terminase motor of phage λ and still yield infectious virions, and the conditions under which this can be efficiently performed. Using a packaging strategy developed in our laboratory of building phage λ from scratch, together with plaque assay monitoring, we have been able to show that the terminase motor of phage λ is able to produce infectious particles with up to 110 % of the wild-type (WT) λ-DNA length. However, the phage production rate, and thus the infectivity, decreased exponentially with increasing DNA length, and was a factor of 10 1 3 lower for the 110 % λ-DNA phage. Interestingly, our in vitro
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study