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Author Toth, I. ♦ Endresz, Valeria ♦ Karcagi, Z. I. Ld Iko ♦ Duda, Erno
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Human Interferon ♦ Mouse Cell ♦ Interferon-fl Gene ♦ Exogenous Interferon Gene ♦ Interferon-at Gene ♦ Interferon Gene ♦ Human Interferon Production ♦ Interferon-specific Mrna ♦ Similar Copy Number ♦ Final Level ♦ Flanking Region ♦ Mouse Lmtk Cell ♦ Human Interferon-fl Gene ♦ Translational Orpost-translational Mechanism Ight ♦ Similar Amount ♦ Transfected Cell ♦ Transfected Ceils ♦ Hybrid Interferon ♦ Human Interferon-cq Gene
Abstract Human interferon-at and interferon-fl genes with their flanking regions were introduced into mouse LMTK- cells. Although transfected cells contained the interferon genes with a similar copy number and produced a similar amount of interferon-specific mRNA, cells containing the human interferon-fl gene secreted about 10 times more human interferon than cells transfected with e human interferon-cq gene. When the coding region of the interferon-fl gene was replaced by that of the interferon-at gene (hybrid interferon fl/a gene), the human interferon production of transfected ceils fell by approx, one order of magnitude. These results show that in the case of exogenous interferon genes a translational orpost-translational mechanism ight significantly affect he final level of human interferons, resulting i higher titres of interferon-fl than of interferon-a.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Publisher Date 1988-01-01