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Author Trask, Douglas K. ♦ Domann, Frederick E.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Athymic Mouse ♦ Sodium-iodide Symporter ♦ Radioiodide Administration ♦ Cell Line ♦ Gene Therapy ♦ Ad-nis Treatment ♦ Clonogenic Survival Assay ♦ Iodide Accumulation ♦ Vitro Hnscc Cell Survival ♦ Squamous Cell Carcinoma ♦ Ad-nis Exhibit Significant Amount ♦ Iodide Delivery ♦ Vivo Cytotoxicity ♦ Iodide Administration ♦ Ni Gene Transfer ♦ Ni Gene ♦ Nis-expressing Adenovirus ♦ Nonthyroidal Cancer ♦ Tumor Growth Experiment ♦ Hnscc Tumor Formation ♦ Neck Cancer ♦ Ni Gene Delivery ♦ Novel Form ♦ Radioiodide Accumulation ♦ Possible Therapeutic Intervention
Abstract Abstract: Background. Gene therapy that uses delivery of the sodium-iodide symporter (NIS) gene followed by radioiodide administration has been proposed as a novel form of radiotherapy for nonthyroidal cancers. Methods. In vitro [125I] iodide accumulation and efflux from cells was determined after treatment with an NIS-expressing adenovirus (Ad-NIS). A clonogenic survival assay and tumor growth experiment that used athymic mice were used to demon-strate the in vitro and in vivo cytotoxicity of Ad-NIS treatment and [131I] iodide delivery. Results. Head and neck squamous cell carcinoma (HNSCC) cell lines treated with Ad-NIS exhibit significant amounts of radioiodide accumulation and retention. In vitro HNSCC cell survival was significantly diminished after NIS gene delivery followed by administration of [131I] iodide. Moreover, NIS gene transfer/[131I] iodide administration dramatically attenuated HNSCC tumor formation in athymic mice. Conclusions. Our data demonstrate the feasibility of gen-etically targeted radiotherapy by use of the NIS gene as a possible therapeutic intervention in head and neck cancer.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2003-01-01