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Author Sahasrabudhe, Janhavi ♦ Palshikar, Shantanu ♦ Goja, Arun ♦ Kulkarni, Rashekhar
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Ghee Residue ♦ Proteus Spp ♦ Microbial Lipase Production ♦ Lipase Production ♦ Tributyrin Oil ♦ Study Ghee Residue ♦ Solid State Fermentation ♦ Submerged Liquid Fermentation ♦ Dairy Industry ♦ Microbial Lipase ♦ Conventional Synthetic Substrate ♦ Main Objective ♦ Efficient Lipase Production ♦ Known Lipase Producer Bacillus Subtilis ♦ Lipase Yield
Abstract ABSTRACT:- Microbial lipases are commercially important and can be produced by Solid State Fermentation (SSF) as well as by Submerged Liquid Fermentation (SLF). In this study ghee residue, which is one of the largest by-product of dairy industry was used as a substrate. The main objective of the study was to determine whether ghee residue could be used as a substrate over the conventional synthetic substrate for efficient lipase production. Two organisms were used for lipase production, one of them was a known lipase producer – Bacillus subtilis (NCIM 2063) and the other organism belonging to the Proteus spp, was isolated from the soil contaminated with oil. The production of lipase was significantly higher in SSF (ghee residue as substrate) than in SLF (tributyrin oil as substrate). The lipase yield was observed to be 41.27 U/mg by Proteus spp and 35.93 U/mg by B. subtilis in SSF using ghee residue as substrate. In SLF using Tributyrin as substrate, the lipase production by Proteus spp was 20.78 U/mg and 28.63 U/mg by B. subtilis. Keywords:- Lipase, ghee residue, Tributyrin oil, SSF, SLF 1.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study