Thumbnail
Access Restriction
Open

Author Henricson, Anna ♦ Käll, Lukas ♦ Sonnhammer, Erik L. L. ♦ Sonnhammer, E.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Computational Evidence ♦ Novel Transmembrane Topology ♦ Reconciling Experimental ♦ Topology Model ♦ Presenilin Topology ♦ Several Topology Model ♦ Novel Nine-transmembrane Topology ♦ Overall Sequence Homology ♦ Intramembrane-cleaving Aspartyl Protease ♦ Xperimental Topology Study ♦ Computational Sequence Analysis ♦ Homologous Presenilin-like Protein Family ♦ Gene Fusion ♦ Presenilin Family ♦ Putative Active Site Residue
Abstract xperimental topology studies using antibodies or gene fusions. The results from these studies are partly contradictory and consequently several topology models have been proposed. Studies of presenilin -interacting proteins have produced further contradiction, primarily regarding the location of the C-terminus. It is thus impossible to produce a topology model that agrees with all published data on presenilin. We have analyzed the presenilin topology through computational sequence analysis of the presenilin family and the homologous presenilin-like protein family. Members of these families are intramembrane-cleaving aspartyl proteases. Although the overall sequence homology between the two families is low, they share the conserved putative active site residues and the conserved `PAL' motif. Therefore, the topology model for the presenilin -like proteins can give some clues about the presenilin topology. Here we propose a novel nine-transmembrane topology with the C-terminus in the extr
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Publisher Date 2005-01-01