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Author Albin, Roger L. ♦ Kume, Akito
Source CiteSeerX
Content type Text
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Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Abstract 4’-Ethynyl-4-n-[2,3-3H,lpropylbicycloorthobenzoate (13HlEBOB) binding to the GABA, receptor complex was characterized autoradiographically in rat brain and then its binding in human brain was investigated. [3H]EBOB binding was saturable, specific and identified a single population of binding sites. The K, obtained from saturation studies was 4.59 nM. Picrotoxin produced dose-dependent inhibition of [ 3H]EBOB binding and saturation analysis indicated a competitive interaction. Isoguvacine inhibited [3H]EBOB binding with regionally different effects. Bicuculline increased [3H]EBOB binding only in the cerebellar granule cell layer. In human cerebellum, a high level of t3H]EBOB binding sites was seen in the granule cell layer. These results suggest that [3H]EBOB binds to the picrotoxin binding site associated with the GABA, receptor complex, that regional differences in GABA, agonist and antagonist modulation of [3H]EBOB binding reflect underlying regional differences in GABA, receptor subunit composition, and that there is a species difference in GABA, receptor distribution between human and rat cerebellum.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Publisher Date 1994-01-01