Access Restriction

Author Mak, Denise Y. F. ♦ Benson, Gary
Source CiteSeerX
Content type Text
Publisher Imperial College Press
File Format PDF
Language English
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Description In Proceedings of the 5th Asia-Pacific Bioinformatics Conference
Motivation: Standard search techniques for DNA repeats start by identifying small matching words, or seeds, that may inhabit larger repeats. Recent innovations in seed structure include spaced seeds (Ma et al. (2002)) and indel seeds (Mak et al. (2006)) which are more sensitive than contiguous seeds. Evaluating seed sensitivity requires 1) specifying a homology model for alignments and 2) assigning probabilities to those alignments. Optimal seed selection is resource intensive because all alternative seeds must be tested (Li et al. (2006)). Current methods require that the model and its probability parameters be specified in advance. When the parameters change, the entire calculation has to be rerun. Results: We show how to eliminate the need for prior parameter specification by exploiting a simple observation: given a homology model, the alignments hit by a particular seed remain the same regardless of the probability parameters. Only the weights assigned to those alignments change. Therefore, if we know all the hits, we can easily (and quickly) find optimal seeds. We describe an efficient preprocessing step, which is computed once per seed. Then we show several increasingly efficient methods to find the optimal seed when given specific probability parameters. Indeed, we show how to determine exactly which seeds can never be optimal under any set of probability parameters. This leads to the startling observation that out of thousands of seeds, only a handful have any chance of being optimal. We then show how to identify optimal seeds and the boundaries within probability space where they are optimal. Contact:
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2007-01-01