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Author Rossi, Edda De ♦ Aı́nsa, Jose ́ A.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Rv2333c Efflux Pump ♦ Antibiotic Resistance ♦ Mycobacterium Tuberculosis ♦ Stp Protein ♦ Rv2333c Gene ♦ Bovis Bcg ♦ Mic Value ♦ Multicopy Plasmid ♦ Tetracycline Accumulation ♦ Antibiotic Susceptibility Test ♦ Efflux Pump ♦ Tetracycline Resistance ♦ Bovis Bcg Chromosome ♦ Mycobacterium Tuber-culosis ♦ Spectinomycin Tetracycline Efflux Pump ♦ Gene Rv2333c ♦ Mycobacterium Bovis Bcg ♦ Model Organism ♦ Tetracycline Uptake Efflux Experiment
Abstract Objectives: To characterize the efflux pump encoded by the gene Rv2333c from Mycobacterium tuber-culosis, and assess its contribution to intrinsic antibiotic resistance using Mycobacterium bovis BCG as a model organism. Methods: Firstly, the Rv2333c gene was expressed from a multicopy plasmid in M. bovis BCG. Secondly, the gene was inactivated in the chromosome of M. bovis BCG. Antibiotic susceptibility tests and tetracycline uptake/efflux experiments were carried out with the strains mentioned above. Results: When the Rv2333c gene was inactivated in the M. bovis BCG chromosome, there was a decrease in the MIC values of spectinomycin and tetracycline, and an increase in [3H]tetracycline accumulation. When the Rv2333c gene was cloned into a multicopy plasmid, there was an increase in the MIC values of spectinomycin and tetracycline, and a decrease in [3H]tetracycline accumulation. These results indicate that both antibiotics are substrates of the Rv2333c efflux pump, which has been named Stp, for Spectinomycin Tetracycline efflux Pump. Conclusions: The Rv2333c efflux pump (Stp protein) of M. tuberculosis contributes to intrinsic specti-nomycin and tetracycline resistance.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study