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Author Cross-Links, Deoxypyridinoline
Source CiteSeerX
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Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword High-sensitivity Enzyme ♦ Bone Resorption ♦ Collection Method ♦ Reference Group ♦ Sweat Pyd ♦ Urine Free Pyridinolines ♦ Prostate Cancer ♦ Mean Increase ♦ High-sensitivity Immu-noassay ♦ Sweat Volume Marker ♦ Biological Fluid ♦ Analytical Recovery ♦ Gonadotropin-releasing Hormone ♦ Collection Device ♦ Quantitative Analysis ♦ Immunoassay Intra ♦ New Platform Sweat ♦ Pyrilinks Immunoassay ♦ Dpd Cr ♦ Detection Limit ♦ Terminal Cross-linked Peptide ♦ Free Pyridinoline Cross-link ♦ Ntx Cr ♦ Sweat Sample ♦ Detec-tion Limit ♦ Weekly Intraindividual Bi-ological Variability ♦ Flame Atomic Emission ♦ Ion-selective Elec-trode Technique ♦ Postmenopausal Woman ♦ Nonocclusive Skin Patch ♦ Human Subject ♦ Mixed Group ♦ Sweat Pyridinolines ♦ Urinary Pyd Cr
Abstract terminal cross-linked peptides (NTX) have been mea-sured in urine as indices of bone resorption. However, very little is known regarding the excretion of pyridino-lines into other biological fluids. We report a collection device, normalizing analyte, and high-sensitivity immu-noassay for quantitative analysis of free pyridinoline cross-links in sweat. Methods: Flame atomic emission and ion-selective elec-trode techniques were used to measure potassium as a sweat volume marker. The Pyrilinks immunoassay for urine free pyridinolines was optimized to increase sen-sitivity for measurements in sweat. The precision, accu-racy, and detection limit of this assay were character-ized. To assess values and variability of sweat pyridinolines in human subjects, a nonocclusive skin patch was used to collect sweat samples from a reference group and from a mixed group experiencing accelerated bone resorption, postmenopausal women and men re-ceiving gonadotropin-releasing hormone for prostate cancer. Results: The immunoassay intra- and interassay varia-tions were <10 % and <16%, respectively, with a detec-tion limit of 309 pmol/L. Linearity upon dilution and analytical recovery ranged from 93 % to 109 % and 85 % to 122%, respectively. Sweat PYD values normalized to potassium output yielded a weekly intraindividual bi-ological variability of 14.7%. The mean increase in the population experiencing increased bone resorption vs the reference group was 36 % (P <0.05) for sweat PYD/K vs 23–40 % (P <0.05) for urinary PYD/Cr, DPD/Cr, and NTX/Cr. Conclusion: We conclude that this new platform sweat
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