|Subject Domain (in DDC)||Computer science, information & general works ♦ Data processing & computer science|
|Subject Keyword||High-sensitivity Enzyme ♦ Bone Resorption ♦ Collection Method ♦ Reference Group ♦ Sweat Pyd ♦ Urine Free Pyridinolines ♦ Prostate Cancer ♦ Mean Increase ♦ High-sensitivity Immu-noassay ♦ Sweat Volume Marker ♦ Biological Fluid ♦ Analytical Recovery ♦ Gonadotropin-releasing Hormone ♦ Collection Device ♦ Quantitative Analysis ♦ Immunoassay Intra ♦ New Platform Sweat ♦ Pyrilinks Immunoassay ♦ Dpd Cr ♦ Detection Limit ♦ Terminal Cross-linked Peptide ♦ Free Pyridinoline Cross-link ♦ Ntx Cr ♦ Sweat Sample ♦ Detec-tion Limit ♦ Weekly Intraindividual Bi-ological Variability ♦ Flame Atomic Emission ♦ Ion-selective Elec-trode Technique ♦ Postmenopausal Woman ♦ Nonocclusive Skin Patch ♦ Human Subject ♦ Mixed Group ♦ Sweat Pyridinolines ♦ Urinary Pyd Cr|
|Abstract||terminal cross-linked peptides (NTX) have been mea-sured in urine as indices of bone resorption. However, very little is known regarding the excretion of pyridino-lines into other biological fluids. We report a collection device, normalizing analyte, and high-sensitivity immu-noassay for quantitative analysis of free pyridinoline cross-links in sweat. Methods: Flame atomic emission and ion-selective elec-trode techniques were used to measure potassium as a sweat volume marker. The Pyrilinks immunoassay for urine free pyridinolines was optimized to increase sen-sitivity for measurements in sweat. The precision, accu-racy, and detection limit of this assay were character-ized. To assess values and variability of sweat pyridinolines in human subjects, a nonocclusive skin patch was used to collect sweat samples from a reference group and from a mixed group experiencing accelerated bone resorption, postmenopausal women and men re-ceiving gonadotropin-releasing hormone for prostate cancer. Results: The immunoassay intra- and interassay varia-tions were <10 % and <16%, respectively, with a detec-tion limit of 309 pmol/L. Linearity upon dilution and analytical recovery ranged from 93 % to 109 % and 85 % to 122%, respectively. Sweat PYD values normalized to potassium output yielded a weekly intraindividual bi-ological variability of 14.7%. The mean increase in the population experiencing increased bone resorption vs the reference group was 36 % (P <0.05) for sweat PYD/K vs 23–40 % (P <0.05) for urinary PYD/Cr, DPD/Cr, and NTX/Cr. Conclusion: We conclude that this new platform sweat|
|Educational Role||Student ♦ Teacher|
|Age Range||above 22 year|
|Education Level||UG and PG ♦ Career/Technical Study|
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
For any issue or feedback, please write to email@example.com