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Author Pythiosis, Patients ♦ Chindamporn, Ariya ♦ Mootsikapun, Piroon
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Immunodominant Antigen ♦ Pythium Insidiosum ♦ 74-kda Antigen ♦ Western Blot Analysis ♦ Human Pythiosis ♦ Pythiosis Serum Sample ♦ Radical Surgery ♦ Healthy Individual ♦ 74-kda Immunodominant Antigen ♦ Etiologic Agent ♦ Insidiosum Isolates ♦ Cornea Ulcer ♦ Control Serum ♦ Different Geographic Area ♦ Con-ventional Antifungal Drug ♦ Humoral Immune Response ♦ Aquatic Organism Pythium Insidiosum ♦ Different Form ♦ Specific Immunodominant Antigen ♦ Main Treatment ♦ Life-threatening Infectious Disease ♦ Human Patient ♦ Temperate Country ♦ Various Infectious Disease
Abstract The oomycetous, fungus-like, aquatic organism Pythium insidiosum is the etiologic agent of pythiosis, a life-threatening infectious disease of humans and animals that has been increasingly reported from tropical, subtropical, and temperate countries. Human pythiosis is endemic in Thailand, and most patients present with arteritis, leading to limb amputation and/or death, or cornea ulcer, leading to enucleation. Diagnosis of pythiosis is time-consuming and difficult. Radical surgery is the main treatment for pythiosis because con-ventional antifungal drugs are ineffective. The aims of this study were to evaluate the use of Western blotting for diagnosis of human pythiosis, to identify specific immunodominant antigens of P. insidiosum, and to increase understanding of humoral immune responses against the pathogen. We performed Western blot analysis on 16 P. insidiosum isolates using 12 pythiosis serum samples. These specimens were derived from human patients with pythiosis who had different forms of infection and lived in different geographic areas throughout Thailand. We have identified a 74-kDa immunodominant antigen in all P. insidiosum isolates tested. The 74-kDa antigen was also recognized by sera from all patients with pythiosis but not by control sera from healthy individuals, patients with thalassemia, and patients with various infectious diseases, indicating that Western blot analysis could facilitate diagnosis of pythiosis. Therefore, the 74-kDa antigen is a potential
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Publisher Date 2005-01-01