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Author Gonzalez-Juarrero, Mercedes ♦ Kingry, Luke C. ♦ Ordway, Diane J. ♦ Henao-Tamayo, Marcela ♦ Harton, Marisa ♦ Basaraba, All J. ♦ Hanneman, William H. ♦ Orme, Ian M. ♦ Slayden, Richard A.
Source CiteSeerX
Content type Text
File Format PDF
Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Molecular Marker ♦ Mycobacterium Tuberculosis ♦ Immune Response ♦ Disease Progression ♦ Respir Cell Mol Biol ♦ Tuberculosis Infection ♦ Mycobacterium Tuberculosis Infection ♦ And100 Day ♦ Dendritic Cell ♦ Signature Profile ♦ H37rv Strain Ofm ♦ Complexmolecular Event ♦ Aerosol Infection ♦ Immunological Response ♦ Anarrayof Cell Surface Marker ♦ Host Response Tom ♦ Gene Expression Profiling ♦ Whole Lung Gene Expression ♦ Transcriptional Response ♦ Unique Molecular Marker ♦ Frommice Collectedat20 ♦ Mouse Whole Genome Array ♦ Compar-ingnormal Lung Tissue ♦ Bacterial Load ♦ Interferon-associated Gene Family ♦ Global Change ♦ Several Gene Transcript ♦ Predictive Marker ♦ Chemokines Andcytokines
Abstract The complexmolecular events that occur within the host during the establishment of a Mycobacterium tuberculosis infection are poorly defined, thus preventing identification of predictive markers of disease progression and state. To identify such molecular markers during M. tuberculosis infection, global changes in transcriptional response in the host were assessed using mouse whole genome arrays. Bacterial load in the lungs, the lesions associated with infection, and gene expression profiling was performed by compar-ingnormal lung tissue to lungs frommice collectedat20, 40, and100 days after aerosol infection with the H37Rv strain ofM. tuberculosis. Quantitative, whole lung gene expression identified signature profiles defining different signaling pathways and immunological responses characteristic of disease progression. This includes genes representing members of the interferon-associated gene families, chemokines andcytokines,MHC, andNOS2, aswell as anarrayof cell surface markers associated with the activation of T cells, macro-phages, and dendritic cells that participate in immunity to M. tuberculosis infection. More importantly, several gene transcripts encoding proteins that were not previously associatedwith the host response toM. tuberculosis infection, and unique molecular markers associated with disease progression and state, were identified.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Publisher Date 2008-01-01