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Author Bunkin, A. F. ♦ Nurmatov, A. A. ♦ Pershin, S. M. ♦ Khusainova, R. S. ♦ Potekhin, S. A.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword ATOMIC AND MOLECULAR PHYSICS ♦ AQUEOUS SOLUTIONS ♦ CHYMOTRYPSIN ♦ COOLING ♦ DNA ♦ HEATING ♦ HYDROGEN PEROXIDE ♦ HYDROXIDES ♦ LASER RADIATION ♦ LASER SPECTROSCOPY ♦ MICROWAVE RADIATION ♦ MOLECULES ♦ PHOTONS ♦ RESOLUTION ♦ RESONANCE ♦ SCATTERING ♦ SIGNALS ♦ SPECTRA ♦ SPECTROMETERS ♦ BOSONS ♦ DISPERSIONS ♦ ELECTROMAGNETIC RADIATION ♦ ELEMENTARY PARTICLES ♦ ENZYMES ♦ HOMOGENEOUS MIXTURES ♦ HYDROGEN COMPOUNDS ♦ HYDROLASES ♦ MASSLESS PARTICLES ♦ MEASURING INSTRUMENTS ♦ MIXTURES ♦ NUCLEIC ACIDS ♦ ORGANIC COMPOUNDS ♦ OXYGEN COMPOUNDS ♦ PEPTIDE HYDROLASES ♦ PEROXIDES ♦ PROTEINS ♦ RADIATIONS ♦ SERINE PROTEINASES ♦ SOLUTIONS ♦ SPECTROSCOPY
Abstract The four-photon laser radiation scattering spectra are obtained in the submillimetre range (75-95 cm{sup -1}) for deionised water, aqueous solutions of DNA and {alpha}-chymotrypsin protein. Narrow resonances are recorded whose frequencies coincide (within the resolution power of a spectrometer) with rotational frequencies in the ground electronic state and vibrational state of ortho and para isomers of H{sub 2}O molecule in a gas phase and with the frequencies of the lines of H{sub 2}O{sub 2} and OH{sup -} molecules. It is shown that the resonance contribution of the rotational lines of ortho isomers of H{sub 2}O to the signal of four-photon scattering of native solutions of biopolymers increases by a factor of at least 8 compared to their contribution to the scattering signal in water, and becomes considerably larger than the contribution from the paraisomer lines. Denaturation of DNA after heating and cooling of the solution leads to the disappearance of such selectivity. (special issue devoted to the 25th anniversary of the a.m. prokhorov general physics institute)
ISSN 10637818
Educational Use Research
Learning Resource Type Article
Publisher Date 2007-10-31
Publisher Place United States
Journal Quantum Electronics
Volume Number 37
Issue Number 10


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