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Author Monjo, Marta ♦ Ramis, Joana M. ♦ Quadros, Paulo A. ♦ Coelho, Catarina C. ♦ Córdoba, Alba
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher MDPI AG
File Format PDF
Date Created 2018-09-12
Copyright Year ©2018
Language English
Subject Domain (in LCC) QD1-999
Subject Keyword Chemistry ♦ Dissolution in simulated gastric fluid ♦ Safety evaluation ♦ Science ♦ Oral care ♦ EU cosmetics regulation ♦ Hydroxyapatite nanoparticles ♦ In vitro biocompatibility ♦ Human gingival epithelium
Abstract Hydroxyapatite nanoparticles (HAP-NP) are incorporated in oral care products such as toothpastes and mouthwashes to treat dental sensitivity or to promote enamel remineralisation. Despite the good performance of HAP-NP in this application, it is important to ensure its safety for consumers. For that reason, the Scientific Committee on Consumer Safety (SCCS) evaluated the safety of HAP-NP as an oral care ingredient, but the issued opinion was not completely conclusive and the SCCS recommended that additional tests should be performed. Here, we used a commercially available human gingival epithelium (HGE) as a non-animal alternative and MTT cell viability, LDH activity, and IL-1alpha production were evaluated after 3.1% HAP-NP treatment for 10 min, 1 h, and 3 h. Moreover, the absorption of HAP-NP in the gingival tissue was assessed by transmission electron microscopy (TEM) analysis. Finally, the dissolution behaviour of HAP-NP in simulated gastric fluid was also investigated. No deleterious effect was observed for HGE tissues incubated with HAP-NP for all time-points and parameters evaluated. Moreover, a complete dissolution of 3.1% HAP-NP in simulated gastric fluid was observed after 7.5 min at 37 °C. In conclusion, our results evidence the safety of HAP-NP for oral care products with the use of an in vitro replacement alternative for human gingival epithelium and a simulated gastric fluid assay.
ISSN 20799284
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2018-09-01
e-ISSN 20799284
Journal Cosmetics
Volume Number 5
Issue Number 3
Starting Page 53


Source: Directory of Open Access Journals (DOAJ)