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Author Planatscher, Hannes ♦ Joos, Thomas O. ♦ Filomena, Angela ♦ Guenther, Anna ♦ Meyer, Hannelore ♦ Terradot, Laurent ♦ Schneiderhanmarra, Nicole ♦ Topin, Francois ♦ She, Joseph ♦ Gerhard, Markus ♦ Formichella, Luca
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher MDPI AG
File Format PDF
Date Created 2017-10-03
Copyright Year ©2017
Language English
Subject Domain (in LCC) QR1-502
Subject Keyword Helicobacter pylori ♦ FliD ♦ Evalution™ ♦ Multiplex serology ♦ Science ♦ Microbiology ♦ Microfluidic
Abstract Infection with Helicobacter pylori (H. pylori) occurs in 50% of the world population, and is associated with the development of ulcer and gastric cancer. Serological diagnostic tests indicate an H. pylori infection by detecting antibodies directed against H. pylori proteins. In addition to line blots, multiplex assay platforms provide smart solutions for the simultaneous analysis of antibody responses towards several H. pylori proteins. We used seven H. pylori proteins (FliD, gGT, GroEL, HpaA, CagA, VacA, and HP0231) and an H. pylori lysate for the development of a multiplex serological assay on a novel microfluidic platform. The reaction limited binding regime in the microfluidic channels allows for a short incubation time of 35 min. The developed assay showed very high sensitivity (99%) and specificity (100%). Besides sensitivity and specificity, the technical validation (intra-assay CV = 3.7 ± 1.2% and inter-assay CV = 5.5 ± 1.2%) demonstrates that our assay is also a robust tool for the analysis of the H. pylori-specific antibody response. The integration of the virulence factors CagA and VacA allow for the assessment of the risk for gastric cancer development. The short assay time and the performance of the platform shows the potential for implementation of such assays in a clinical setting.
ISSN 22277382
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2017-10-01
e-ISSN 22277382
Journal Proteomes
Volume Number 5
Issue Number 4
Starting Page 24

Source: Directory of Open Access Journals (DOAJ)