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Author Shelud'ko, Nikolay S. ♦ Girich, Ulyana V. ♦ Lazarev, Stanislav S. ♦ Vyatchin, Ilya G.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ACETONE ♦ ACTIN ♦ ATP ♦ DEPOLYMERIZATION ♦ ELECTRON MICROSCOPY ♦ FILAMENTS ♦ FLUORIDES ♦ MUSCLES ♦ MUSSELS ♦ MYOSIN ♦ PHOSPHATES ♦ POLYMERIZATION ♦ POTASSIUM CHLORIDES ♦ RABBITS ♦ TROPOMYOSIN
Abstract We have developed a method of obtaining natural actin from smooth muscles of the bivalves on the example of the Crenomytilus grayanus catch muscle. The muscles were previously rigorized to prevent a loss of thin filaments during homogenization and washings. Thin filaments were isolated with a low ionic strength solution in the presence of ATP and sodium pyrophosphate. Surface proteins of thin filaments-tropomyosin, troponin, calponin and some minor actin-binding proteins-were dissociated from actin filaments by increasing the ionic strength to 0.6 M KCL. Natural fibrillar actin obtained in that way depolymerizes easily in low ionic strength solutions commonly used for the extraction of Straub-type actin from acetone powder. Purification of natural actin was carried out by the polymerization–depolymerization cycle. The content of inactivated actin remaining in the supernatant is much less than at a similar purification of Straub-type actin. A comparative investigation was performed between the natural mussel actin and the Straub-type rabbit skeletal actin in terms of the key properties of actin: polymerization, activation of Mg-ATPase activity of myosin, and the electron-microscopic structure of actin polymers. -- Highlights: •We developed method of repolymerizable invertebrate smooth muscle actin obtaining. •Our method does not involve use of denaturating agents, which could modify proteins. •Viscosity and polymerization rate of actin, gained that way, is similar to Straub one. •Electron microscopy showed that repolymerized mussel actin is similar to Straub one. •Repolymerized mussel actin has greater ATPase activating capacity, than Straub actin.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2016-05-27
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 474
Issue Number 2


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