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Author Prathap ♦ Chilka, Pallavi ♦ Patlolla, Prathap Reddy ♦ Datta, Bhaskar
Source IIT Gandhinagar
Content type Text
Publisher Royal Society of Chemistry
Language English
Abstract A novel dimeric carbocyanine dye is found to recognise G-quadruplex structures selectively compared to mixed sequence or double-stranded DNA molecules. The dye exhibits a preference for G3-bearing parallel quadruplexes and results in a nearly 1400-fold enhancement compared to the absence of DNA and a nearly 30-fold fluorescence enhancement compared to duplex and single stranded DNA molecules. Study of the dye binding with various quadruplex-forming sequences points towards an end-stacking mechanism of interaction. The number of G-quartets and spacer sequences along with the identity of monovalent ions strongly influence the interaction of quadruplexes with the dye.
ISSN 20462069
Learning Resource Type Article
Publisher Date 2016-09-01
Journal RSC Advances
Volume Number 6
Issue Number 90
Starting Page 87400
Ending Page 87404


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