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Author Purushothaman, Gayathri ♦ Thiruvenkatam, Vijay
Source IIT Gandhinagar
Content type Text
Language English
Subject Keyword 3D Structure ♦ Tetraspanin
Abstract Tetraspanins are family of small membrane proteins and they are involved in multitude of biological process. Structurally theyare characterized by having four transmembrane domains, short inner and outer loops, one large extra cellular loop containsCCG motif and N and C terminal. Iconic features of these proteins are formation of Tetraspanin Enriched Micro domains(TEMs) by interacting among themselves and with other transmembrane and cytosolic proteins. These domains provide asignaling platform for many important cellular functions such as immune response induction, fertilization, viral infection,maintenance of skin integrity and malignant process. Tetraspanin CD151 is frequently over expressed on cancer cells and isfunctionally linked to cancer metastasis. CD151 forms direct and stable and interaction with integrin molecules and regulatesthe cellular functions. Increasing evidence emerging from in vitro, in vivo and clinical analyses associates that CD151partnership with integrins α6β1 and α6β4, modulates different stages in cancer such as tumor cell growth, metastasis anddrug sensitivity in various types of cancer. The importance of CD151 signposts that targeting CD151 could be a promisingtherapy in cancer and other viral infections. Even though evidences are there for the CD151-Integrin interactions, themechanisms and mode of the interactions are not yet known. The structural and functional characteristics of CD151 via thethree dimensional structure of the protein would pave way for understanding the TEMs of CD151. By considering thechallenges in the structural determination of membrane protein, we expressed and purified the specific portion of CD151 inbacterial system. The expressed portion includes large extra cellular loop alone as well along with the one transmembranedomain and C-Terminal which expected to be have interaction with Integrins and other partner proteins. The expressedrecombinant protein confirmed by western blot and MALDI. The activity of the recombinant protein will be assessed bycholesterol binding assays followed the protein will be experiment to crystallization.
Spatial Coverage International Convention Centre, Hyderabad, IN
Learning Resource Type Presentation
Publisher Date 2017-08-21