Access Restriction

Author Alsanjary, R. A.
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher University of Mosul, College of Veterinary Medicine
File Format HTM / HTML
Date Created 2010-01-31
Copyright Year ©2009
Language Arabic ♦ English
Subject Domain (in LCC) SF600-1100
Subject Keyword Agriculture ♦ Animal culture ♦ Veterinary medicine
Abstract To differentiate the beef from other types of meat consumed by human, DNA markers based on polymerase chain reaction restriction fragment length polymorphism technique is performed by using universal primers designed on mitochondrial cytochrome b gene to obtain amplified band 359 bp, then digested with some of restriction enzymes like Tru91, RsaI, Hinf I, Hae III, Alu I, Taq I, Mob I. The result revealed that, the Hinf I enzyme produce three bands 198, 117, 44 bp and the Hae III enzyme revealed two band 285, 74 bp, the Alu I enzyme also produced two band but the molecular weight are 190, 169 bp. The other enzymes did not reveal any digestion of the amplified bands and this result is a characteristic unique to beef compared with other types of meat when using same enzymes.
ISSN 20711255
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2009-01-01
e-ISSN 16073894
Journal Iraqi Journal of Veterinary Sciences
Volume Number 23 (Arabic)
Issue Number 1
Page Count 4
Starting Page 43
Ending Page 46

Source: Directory of Open Access Journals (DOAJ)